Summary: The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein of interest by proximity-dependent biotinylation. Here. we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry. suitable to specifically map the content of autophagosomes and potentially other short-lived endomembrane transport vesicles without the... https://macorners.shop/product-category/hoodie-and-sweatpants-set/